The non-enzymatic glucose sensor has garnered significant interest in the fabrication of blood glucose sensors due to its longer lifespan compared to enzymatic biosensors, which eliminate biological enzymes in glucose detection. This study employed Aluminium (Al) as a material to fabricate a non-enzymatic electrode using conventional photolithography techniques due to its low cost and good conductivity. The fabricated Aluminium electrode demonstrated a response to glucose oxidation, as evidenced by the formation of oxidation and reduction peaks in the Cyclic Voltammetry (CV) measurement. The oxidation peaks occurred at around 0.623 V and 0.68 V when the glucose concentration was 1mM and 8mM, respectively. Although the peak anodic potential was higher than the usual value at around 0.4 V, the low cost of Aluminium could compensate for this higher detection limit. Furthermore, the effect of glucose on the conventional Gold (Au) electrode and Glassy Carbon Electrode (GCE) was investigated under different pH conditions, glucose concentrations, and the presence of Phosphate Buffer through Cyclic Voltammetry (CV) measurements. Both working electrodes demonstrated that the glucose concentration did not affect the peak oxidation and reduction potential in the presence of Phosphate Buffer. Additionally, GCE demonstrated a better glucose response under pH 5 and pH 8 buffered solutions compared to neutral pH 7 buffered solutions.